[박상규] C2C12 differentiation into myocyte

This topic has 0 replies, 1 voice, and was last updated 15 years, 10 months ago by .

  • Author
    Posts
    • #18973

      Anonymous
      Inactive

      C2C12 differentiation into myocyte

       

       

      1.     Seed the cells into 12 well plate and culture the cells with DMEM containing 10% FBS

      2.     Culture till confluent and culture the cells 2 days more at the confluent status

      3.     Change the media with DMEM containing 2% horse serum and culture for 2 days

      4.     Change the media at every 2 day and culture for 2 days

      5.     Repeat step 4 till differentiation rate of C2C12 cells is 80% è you can see the differentiation rate by examination of your cells under the light microscope

      è cell are differentiated, you can see the multinucleated myotube

Viewing 0 reply threads

You must be logged in to reply to this topic.

CONTACT US

We're not around right now. But you can send us an email and we'll get back to you, asap.

Sending

©2010-2024 Medicinal Bioconvergence Research Center. All rights reserved.

Log in with your credentials

Forgot your details?