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수많은 단백질들의 분자구조를 실험을 통해 직접 규명하는 것은 시간과 노력 및 예산이 무척 많이 드는 일입니다. 이것을 컴퓨터 시뮬레이션을 이용하여 예측할 수 있다면 크게 도움이 되겠지요. 최근에는 단순히 컴퓨터를 이용하는...

Purification of IgG from serum 1.     Add 1ml of serum to 10ml of IP buffer2.     Add 200ul protein A(mouse serum : protein G, 50ul) agarose to serum/ IP buffer mixture(conical...

Preparation of Nuclei from mouse Liver stocks1.     2.5 M sucrose (85%,w/v) : to make 1 liter, dissolve 850g of  sucrose in distilled water and adjust to a total volume of...

Preparation of cytoplasmic extracts and gel-filtration from 293 cells  1.     spin down the cells at 600Xg for 5min and wash with 1X ice cold PBS( 150 mm 5dishes)2.     resuspend the...

Isolation of total RNA from cell line  Materials-Trizol : cat# 15596-026(invitrogen)-DEPC treated & autoclaved water(0.2% DEPC)-Autoclaved blue & yellow tip & eppendorf tube-Isopropanol, cold 70% ethanol-PBS 1.     Wash the cells in...

Isolation of total RNA from cell line  Materials-Trizol : cat# 15596-026(invitrogen)-DEPC treated & autoclaved water(0.2% DEPC)-Autoclaved blue & yellow tip & eppendorf tube-Isopropanol, cold 70% ethanol-PBS 1.     Wash the cells in...

Isolation of total RNA from cell line  Materials-Trizol : cat# 15596-026(invitrogen)-DEPC treated & autoclaved water(0.2% DEPC)-Autoclaved blue & yellow tip & eppendorf tube-Isopropanol, cold 70% ethanol-PBS 1.     Wash the cells in...

Purification of IgG from serum (1ml) 1.     Dilute serum with 1XPBS 1:12.     Equilibrate the protein A agarose (rabbit serum) or protein G agarose (mouse ascites) (600ul) with 1ml of 1X...

Purification of IgG from serum (1ml) 1.     Dilute serum with 1XPBS 1:12.     Equilibrate the protein A agarose (rabbit serum) or protein G agarose (mouse ascites) (600ul) with 1ml of 1X...

Purification of IgG from serum (1ml) 1.     Dilute serum with 1XPBS 1:12.     Equilibrate the protein A agarose (rabbit serum) or protein G agarose (mouse ascites) (600ul) with 1ml of 1X...

Antibody production from Rabbit                                                             2005.3.03        Arthur1.     Purchase NewZealand White rabbit (male, 1kg)2.     Adapt rabbits for 3-7days3.     Pick up the blood (200-300ul) from ear vein using 1ml syringe and store...

Antibody production from Rabbit                                                             2005.3.03        Arthur1.     Purchase NewZealand White rabbit (male, 1kg)2.     Adapt rabbits for 3-7days3.     Pick up the blood (200-300ul) from ear vein using 1ml syringe and store...

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