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재미있는 논문이 있어 공유하려고 글 올립니다. 올해 3월에 나논 논문인데요. tRNA 자체가 signaling molecule로 기능한다는 점을 증명했다는 점에서  시사하는 바가 큰 논문이라 생각됩니다. 논문에서 밝힌 cytochrome c와 결합하는 tRNA는 총...

Northern Hybridization for tRNA                                                            Sep. 24. 2001.Joong-Won Lee   *Sample preparation -Total RNA preparation    a.RNA prep. by Trizol method(1)   b.The pellet is dissolved in appropreate volume of 10mM NaOAc(pH4.5) 1mM EDTA or...

Northern Hybridization for tRNA                                                            Sep. 24. 2001.Joong-Won Lee   *Sample preparation -Total RNA preparation    a.RNA prep. by Trizol method(1)   b.The pellet is dissolved in appropreate volume of 10mM NaOAc(pH4.5) 1mM EDTA or...

Northern Hybridization for tRNA                                                            Sep. 24. 2001.Joong-Won Lee   *Sample preparation -Total RNA preparation    a.RNA prep. by Trizol method(1)   b.The pellet is dissolved in appropreate volume of 10mM NaOAc(pH4.5) 1mM EDTA or...

Northern Blot for tRNA  RNA extraction All steps should be carried out in the cold. 1. Grow MEF cells in five 100-mm dishes. 2. Wash cells 2x with cold PBS and harvest by...

Northern Blot for tRNA  RNA extraction All steps should be carried out in the cold. 1. Grow MEF cells in five 100-mm dishes. 2. Wash cells 2x with cold PBS and harvest by...

Northern Blot for tRNA  RNA extraction All steps should be carried out in the cold. 1. Grow MEF cells in five 100-mm dishes. 2. Wash cells 2x with cold PBS and harvest by...

Detact of tRNA aminoacylation and deacylationin p38KO MEF cells containing 35-S Methionine.2001 Joong-Won Lee    1.prepare methionine-free DMEM containing 10% dialyzed FBS. 2.washing the dish with 1X PBS two times and change...

Detact of tRNA aminoacylation and deacylationin p38KO MEF cells containing 35-S Methionine.2001 Joong-Won Lee    1.prepare methionine-free DMEM containing 10% dialyzed FBS. 2.washing the dish with 1X PBS two times and change...

Detact of tRNA aminoacylation and deacylationin p38KO MEF cells containing 35-S Methionine.2001 Joong-Won Lee    1.prepare methionine-free DMEM containing 10% dialyzed FBS. 2.washing the dish with 1X PBS two times and change...

흥미로운 논문이네요.. Download : tRNA-mediated splicingDownload : 0802941105v1

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